Evidence that the cis preference of the Tn5 transposase is caused by nonproductive multimerization.

doi:10.1101/gad.8.19.2363

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Article

Evidence that the cis preference of the Tn5 transposase is caused by nonproductive multimerization.

Genes & development - 1 Oct 1994

Weinreich M D, Gasch A, Reznikoff W S

Abstract excerpt

The transposase (Tnp) of the bacterial transposon Tn5 acts 50- to 100-fold more efficiently on elements located cis to the site of its synthesis compared with those located in trans. In an effort to understand the basis for this cis preference, we have screened for Tnp mutants that exhibit increa...

Topics

Base Sequence
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Molecular Sequence Data
Mutation
Nucleotidyltransferases
Polymerase Chain Reaction
Protein Conformation
Transcriptional Activation
Transposases

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