Article
Introduction of specific point mutations into RNA polymerase II by gene targeting in mouse embryonic stem cells: evidence for a DNA mismatch repair mechanism.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Steeg C M, Ellis J, Bernstein A
Abstract excerpt
We have introduced two specific point mutations, located 20 base pairs apart, into the endogenous murine gene that encodes the largest subunit of RNA polymerase II (RPII215). The first mutation conferred resistance to the mushroom toxin alpha-amanitin (amar), and the second mutation generated a r...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Cell Line
- Clone Cells
- DNA
- DNA Repair
- Embryo, Mammalian
- Macromolecular Substances
- Mice
- Molecular Sequence Data