Article
Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.
Nucleic acids research - 25 May 1990
Sizemore C, Wissmann A, Gülland U, Hillen W
Abstract excerpt
A saturating oligonucleotide-directed mutagenesis of both tet operators in the tet regulatory sequence was performed yielding mutants with four identical base pair exchanges at equivalent positions in the four tet operator half sides. The mutants were cloned between bipolar lacZ and galK indicator genes on a multicopy plasmid allowing the quantitative analysis of their effects in vivo. In the absence of Tet...
Topics
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Operator Regions, Genetic