Article
Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor.
The EMBO journal - 1 Dec 1991
Wissmann A, Baumeister R, Müller G, Hecht B, Helbl V, Pfleiderer K, Hillen W
Abstract excerpt
Each of 22 amino acids in the proposed alpha-helix-turn-alpha-helix operator binding motif of the Tn10 encoded Tet repressor was replaced by alanine and one residue was replaced by valine to determine their role in tet operator recognition by a 'loss of contact' analysis with 16 operator variants. One class of amino acids consisting of T27 and R28 in the first alpha-helix and L41, Y42, W43 and H44 in the...
Topics
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Blotting, Western
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic
- Plasmids
- Protein Conformation