Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites.

Abstract excerpt

A series of computer simulations of gel patterns assuming non-cooperative binding of a protein to two targets on the same DNA fragment was performed and applied to interprete gel mobility shift experiments of Tet repressor-tet operator binding. While a high binding affinity leads to the expected...

Topics

• Binding Sites
• Computer Simulation
• DNA, Bacterial
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli
• Genetic Variation
• Operator Regions, Genetic
• Repressor Proteins