Article
Biochemical and genetic analysis of operator contacts made by residues within the beta-sheet DNA binding motif of Mnt repressor.
The EMBO journal - 1 Jan 1992
Knight K L, Sauer R T
Abstract excerpt
Residues 2, 6, 8 and 10 of Mnt repressor are the major determinants of operator DNA binding and recognition. Here, we investigate the interaction of wild-type Mnt and mutants bearing the Arg2----Lys, His6----Ala, Asn8----Ala and Arg10----Lys mutations with operator DNA modified by methylation or by symmetric base substitutions. The wild-type pattern of methylation interference is altered in specific ways for each...
Topics
- Base Sequence
- Binding Sites
- Coliphages
- DNA Mutational Analysis
- DNA-Binding Proteins
- Escherichia coli
- Methylation
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic