Proximity probing of Tet repressor to tet operator by dimethylsulfate reveals protected and accessible functions for each recognized base-pair in the major groove.

Abstract excerpt

We have tracked the path of Tet repressor across the major groove in the complex with tet operator. This was done by a methylation protection analysis of nine tet operator mutants containing replacements by a G residue of each nucleotide in base-pairs important for Tet repressor recognition. We demonstrated sequence-specific binding of Tet repressor to these operator mutants using DNA retardation assays and the...

Topics

• Base Composition
• Base Sequence
• DNA
• Hydroxyl Radical
• Methylation
• Molecular Sequence Data
• Mutation
• Operator Regions, Genetic
• Repressor Proteins
• Sulfuric Acid Esters