Article
Effect of irradiation and mutagenic chemicals on the generation of ADH2- and ADH4-constitutive mutants in yeast: the inducibility of Ty transposition by UV and ethyl methanesulfonate.
Mutation research - 1 Mar 1990
Morawetz C, Hagen U
Abstract excerpt
A strain defective in fermentation due to a deletion in the ADH1 gene was used to generate revertants which are constitutive mutants of the genes ADH2 and ADH4. By analyzing the DNA of the mutants we determined the frequency of Ty insertions into the promoter region of these genes. We found an increase in transposition after UV irradiation and treatment with ethyl methanesulfonate (EMS). Chemical inhibition of...
Topics
- Alcohol Oxidoreductases
- Antimycin A
- DNA Transposable Elements
- DNA, Fungal
- Drug Resistance, Microbial
- Ethyl Methanesulfonate
- Mathematics
- Mutagens
- Mutation
- Saccharomyces cerevisiae
- Ultraviolet Rays