Article
Single-step selection for Ty1 element retrotransposition.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1991
Curcio M J, Garfinkel D J
Abstract excerpt
The yeast retrotransposon Ty1 has been tagged with a reporter gene that allows selection of RNA-mediated transposition events and is applicable to the study of retroelements in other organisms. The reporter gene is a yeast HIS3 gene interrupted by an artificial intron (AI) in the antisense orientation. The HIS3AI sequences were inserted into a Ty1 element such that the intron is on the sense strand of the Ty1...
Topics
- Blotting, Northern
- DNA Transposable Elements
- Genes, Fungal
- Introns
- Phenotype
- Plasmids
- RNA, Fungal
- Saccharomyces cerevisiae