Article
Yeast mutants with increased bacterial transposon Tn5 excision.
Yeast (Chichester, England) - 1 Jan 1991
Gordenin D A, Proscyavichus Y Y, Malkova A L, Trofimova M V, Peterzen A
Abstract excerpt
Five complementing recessive mutations that exhibit increased bacterial transposon Tn5 precise excision in yeast Saccharomyces cerevisiae were obtained by ethylmethanesulfonate treatment. One of these mutations (tex1) was submitted to extensive genetic analysis. tex1 is a recessive temperature-sensitive mutation resulting in a 20-100-fold increase in Tn5 excision. It also has increased frequencies of ochre...
Topics
- Alleles
- DNA Transposable Elements
- Mutagenesis, Insertional
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic