Article
Electroporation-stimulated recombination in yeast.
Yeast (Chichester, England) - 1 Nov 1991
Higgins D R, Strathern J N
Abstract excerpt
Saccharomyces cerevisiae cells treated by high voltage and made transformation-competent (electroporation) are also made hyper-recombinational as determined by an assay that measures interchromosomal mitotic recombination between chromosome III homologs, each containing mutant heteroallelic copies of the trp1 and his3 genes. There is a 10-fold stimulation of Trp+ and 21-fold stimulation of His+ prototrophs....
Topics
- Alleles
- DNA, Fungal
- Electric Stimulation
- Mutation
- Plasmids
- Recombination, Genetic
- Saccharomyces cerevisiae
- Transformation, Genetic
