A genetic locus in mutant poliovirus genomes involved in overproduction of RNA polymerase and 3C proteinase.

doi:10.1016/0042-6822(90)90104-y

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Article

A genetic locus in mutant poliovirus genomes involved in overproduction of RNA polymerase and 3C proteinase.

Virology - 1 Feb 1990

Dewalt P G, Blair W S, Semler B L

Abstract excerpt

A mutagenic oligonucleotide cassette was used to introduce single and tandem amino acid substitutions into the proteinase 3C coding region of an infectious poliovirus type 1 cDNA. The sites targeted for mutagenesis, residues 60, 61, and 66, are located within a putative helical loop structure whi...

Topics

3C Viral Proteases
Chromosome Mapping
Cysteine Endopeptidases
DNA
DNA-Directed RNA Polymerases
Genes, Viral
HeLa Cells
Mutation
Poliovirus
RNA, Viral
Structure-Activity Relationship
Viral Proteins

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