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Article

Engineering a novel endopeptidase based on SARS 3CL(pro).

BioTechniques - 1 Dec 2009

Kuo Chih-Jung, Shih Yan-Ping, Kan Daphne, Liang Po-Huang

Abstract excerpt

A 3C-like protease (3CLpro) from the severe acute respiratory syndrome-coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln [downward arrow](Ser, Ala, Gly) sequences. In this study, we developed a mutant 3CLpro (T25G) with an expanded S1' space that demonstrates 43.5-fold better k(cat)/K(m) compared with wild-type in cleaving substrates...

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Share to Coronavirus 3C Proteases
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 20041855
DOI: 10.2144/000113303
PMCID: PMC7081961

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