Article
Analysis of putative active site residues of the poliovirus 3C protease.
Virology - 1 Apr 1991
Kean K M, Teterina N L, Marc D, Girard M
Abstract excerpt
It was recently suggested that the picornavirus 3C proteases are homologous to the chymotrypsin-like serine proteases. The two structural models proposed differ in one of the postulated active site residues, Glu/Asp71 or Asp85. We changed Glu71 of the poliovirus type 1 protease to Asp or Gln and Asp85 to Glu by oligonucleotide-directed site-specific mutagenesis of an infectious cDNA, and attempted to recover...
Topics
- 3C Viral Proteases
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Cysteine Endopeptidases
- DNA, Viral
- HeLa Cells
- Humans
- Kinetics
- Methionine
- Molecular Sequence Data