Article
Substitutions in the protease (3Cpro) gene of poliovirus can suppress a mutation in the 5' noncoding region.
Journal of virology - 1 Feb 1990
Andino R, Rieckhof G E, Trono D, Baltimore D
Abstract excerpt
The poliovirus mutant 5NC-11 has a 4-base insertion at position 70 within the 5' untranslated region and is deficient in RNA synthesis. Revertants from 5NC-11 were isolated, showing a partial recovery of wild-type levels of RNA synthesis. The 5' noncoding region of those revertants contained the mutation intact; mix-and-match experiments with the cDNA from these revertants revealed that a restricted region within...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Genes, Viral
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Peptide Hydrolases
- Phenotype