Article
Enzymatic activity of poliovirus RNA polymerases with mutations at the tyrosine residue of the conserved YGDD motif: isolation and characterization of polioviruses containing RNA polymerases with FGDD and MGDD sequences.
Journal of virology - 1 Jan 1993
Jablonski S A, Morrow C D
Abstract excerpt
The poliovirus RNA-dependent RNA polymerase (3Dpol) shares a region of homology with all RNA polymerases, centered around the amino acid motif YGDD, which has been postulated to be involved in the catalytic activity of the enzyme. Using oligonucleotide site-directed mutagenesis, we substituted th...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cells, Cultured
- Conserved Sequence
- Escherichia coli
- Genes, pol
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Poliovirus
- RNA-Dependent RNA Polymerase
- Sequence Homology, Amino Acid