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Article

Local de novo assembly of RAD paired-end contigs using short sequencing reads.

PloS one - 13 Apr 2011

Etter Paul D, Preston Jessica L, Bassham Susan, Cresko William A, Johnson Eric A

Abstract excerpt

Despite the power of massively parallel sequencing platforms, a drawback is the short length of the sequence reads produced. We demonstrate that short reads can be locally assembled into longer contigs using paired-end sequencing of restriction-site associated DNA (RAD-PE) fragments. We use this RAD-PE contig approach to identify single nucleotide polymorphisms (SNPs) and determine haplotype structure in...

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PMID: 21541009
DOI: 10.1371/journal.pone.0018561
PMCID: PMC3076424

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