Article
RAD paired-end sequencing for local de novo assembly and SNP discovery in non-model organisms.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Etter Paul D, Johnson Eric
Abstract excerpt
Restriction-site Associated DNA (RAD) markers are rapidly becoming a standard for SNP discovery and genotyping studies even in organisms without a sequenced reference genome. It is difficult, however, to identify genes nearby RAD markers of interest or move from SNPs identified by RAD to a high-throughput genotyping assay. Paired-end sequencing of RAD fragments can alleviate these problems by generating a set of...
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