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Mass production of SNP markers in a nonmodel passerine bird through RAD sequencing and contig mapping to the zebra finch genome.

Molecular ecology resources - 1 Sept 2013

Bourgeois Yann X C, Lhuillier Emeline, Cézard Timothée, Bertrand Joris A M, Delahaie Boris, Cornuault Josselin, Duval Thomas, Bouchez Olivier, Milá Borja, Thébaud Christophe

Abstract excerpt

Here, we present an adaptation of restriction-site-associated DNA sequencing (RAD-seq) to the Illumina HiSeq2000 technology that we used to produce SNP markers in very large quantities at low cost per unit in the Réunion grey white-eye (Zosterops borbonicus), a nonmodel passerine bird species with no reference genome. We sequenced a set of six pools of 18-25 individuals using a single sequencing lane. This...

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PMID: 23855484
DOI: 10.1111/1755-0998.12137

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