Article
G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Mumby S M, Heukeroth R O, Gordon J I, Gilman A G
Abstract excerpt
Myristoylation of seven different alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) was examined by expressing these proteins in monkey kidney COS cells. Metabolic labeling studies of cells transfected with cytomegalovirus-based expression vectors indicated that [3H]my...
Topics
- Animals
- Cell Line
- Cell Membrane
- Cytomegalovirus
- DNA
- GTP-Binding Proteins
- Genetic Vectors
- Kinetics
- Macromolecular Substances
- Mutation
- Myristic Acid
- Myristic Acids
- Plasmids
- Protein Processing, Post-Translational
- Transfection