Article
Myristoylation of an inhibitory GTP-binding protein alpha subunit is essential for its membrane attachment.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Jones T L, Simonds W F, Merendino J J, Brann M R, Spiegel A M
Abstract excerpt
We transfected COS cells with cDNAs for the alpha subunits of stimulatory and inhibitory GTP-binding proteins, alpha s and alpha i1, respectively, and immunoprecipitated the metabolically labeled products with specific peptide antibodies. Cells were separated into particulate and soluble fractions before immunoprecipitation; [35S]methionine-labeled alpha s and alpha i were both found primarily in the particulate...
Topics
- Adenosine Diphosphate Ribose
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cell Membrane
- DNA
- GTP-Binding Proteins
- Hydroxylamine
- Hydroxylamines
- Macromolecular Substances