Article
The palmitoylation status of the G-protein G(o)1 alpha regulates its activity of interaction with the plasma membrane.
The Biochemical journal - 15 Sept 1994
Grassie M A, McCallum J F, Guzzi F, Magee A I, Milligan G, Parenti M
Abstract excerpt
Plasmids containing cDNAs encoding either the wild-type guanine-nucleotide-binding protein G(o)1 alpha or the palmitoylation-negative cysteine-3-to-serine (C3S) mutant of G(o)1 alpha were transfected into Rat 1 cells, and clones stably expressing immunoreactivity corresponding to these polypeptid...
Topics
- Adenosine Diphosphate
- Animals
- Catalysis
- Cell Membrane
- Cells, Cultured
- Chromatography, Gel
- Cysteine
- Fibroblasts
- GTP-Binding Proteins
- Hydroxylamine
- Hydroxylamines
- Immunoblotting
- Mutation
- Myristic Acid
- Myristic Acids
- Palmitates
- Pertussis Toxin
- Precipitin Tests