Article
Comprehensive analysis of the specificity of transcription activator-like effector nucleases.
Nucleic acids research - 1 Apr 2014
Juillerat Alexandre, Dubois Gwendoline, Valton Julien, Thomas Séverine, Stella Stefano, Maréchal Alan, Langevin Stéphanie, Benomari Nassima, Bertonati Claudia, Silva George H, Daboussi Fayza, Epinat Jean-Charles, Montoya Guillermo, Duclert Aymeric, Duchateau Philippe
Abstract excerpt
A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site. The ability to predict the specificity of targeting is thus highly desirable. Here, we describe the first...
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