Article
Transcription activator-like effector nucleases enable efficient plant genome engineering.
Plant physiology - 1 Jan 2013
Zhang Yong, Zhang Feng, Li Xiaohong, Baller Joshua A, Qi Yiping, Starker Colby G, Bogdanove Adam J, Voytas Daniel F
Abstract excerpt
The ability to precisely engineer plant genomes offers much potential for advancing basic and applied plant biology. Here, we describe methods for the targeted modification of plant genomes using transcription activator-like effector nucleases (TALENs). Methods were optimized using tobacco (Nicotiana tabacum) protoplasts and TALENs targeting the acetolactate synthase (ALS) gene. Optimal TALEN scaffolds were...
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