Article
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
The EMBO journal - 1 Oct 1990
Vankan P, McGuigan C, Mattaj I W
Abstract excerpt
Structure-function relationships in the vertebrate U4-U6 snRNP have been analysed by assaying the ability of mutant RNAs to form U4-U6 snRNPs and to function in splicing complementation in Xenopus oocytes. The mutants define three categories of domain within the RNAs. First, domains which are not essential for splicing. These include regions of U6 which have previously been implicated in the capping and transport...
Topics
- Animals
- Base Sequence
- Cell Nucleus
- Female
- Genetic Complementation Test
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oocytes
- RNA Splicing