Article
Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.
The EMBO journal - 1 Apr 1990
Hamm J, Dathan N A, Scherly D, Mattaj I W
Abstract excerpt
Domains of U1 snRNA which are functionally important have been identified using a splicing complementation assay in Xenopus oocytes. Mutations in, and deletions of, all three of the hairpin loop structures near the 5' end of the RNA are strongly deleterious. Similarly, mutation of the Sm binding...
Topics
- Animals
- Base Sequence
- Chromosome Deletion
- Cloning, Molecular
- Escherichia coli
- Female
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oocytes
- Protein Binding
- RNA Splicing
- RNA, Small Nuclear
- Ribonucleoproteins
- Ribonucleoproteins, Small Nuclear
- Xenopus