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Article

High-resolution melting analysis for bird sexing: a successful approach to molecular sex identification using different biological samples.

Molecular ecology resources - 1 May 2013

Morinha Francisco, Travassos Paulo, Seixas Fernanda, Santos Nuno, Sargo Roberto, Sousa Luís, Magalhães Paula, Cabral João A, Bastos Estela

Abstract excerpt

High-resolution melting (HRM) analysis is a very attractive and flexible advanced post-PCR method with high sensitivity/specificity for simple, fast and cost-effective genotyping based on the detection of specific melting profiles of PCR products. Next generation real-time PCR systems, along with improved saturating DNA-binding dyes, enable the direct acquisition of HRM data after quantitative PCR. Melting...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23433263
DOI: 10.1111/1755-0998.12081

Genes

CHD1

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