Article
Application of high-resolution melting to large-scale, high-throughput SNP genotyping: a comparison with the TaqMan method.
Journal of biomolecular screening - 1 Jul 2010
Martino Alessandro, Mancuso Tommaso, Rossi Anna Maria
Abstract excerpt
Because of the wide use of single-nucleotide polymorphisms (SNPs) as markers of genetic variation, several high-throughput genotyping methods have been developed and applied during the past decades. High-resolution melting (HRM) is a very attractive, advanced, fast, and cost-effective SNP genotyping technology based on the analysis of the melting profile of PCR products, using intercalating fluorescent dyes to...
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