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Article

A flow cytometry-based screening system for directed evolution of proteases.

Journal of biomolecular screening - 1 Mar 2011

Tu Ran, Martinez Ronny, Prodanovic Radivoje, Klein Mathias, Schwaneberg Ulrich

Abstract excerpt

Proteases are industrially important enzymes but often have to be improved for their catalytic efficiency and stabilities to suit applications. Flow cytometry screening technology based on in vitro compartmentalization in double emulsion had been developed and applied on directed evolution of paraoxonase and β-galactosidase. Further advancements of flow cytometry-based screening technologies will enable an...

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PMID: 21335599
DOI: 10.1177/1087057110396361

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