Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity.

doi:10.1073/pnas.1621226114

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Article

Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity.

Proceedings of the National Academy of Sciences of the United States of America - 7 Mar 2017

Terekhov Stanislav S, Smirnov Ivan V, Stepanova Anastasiya V, Bobik Tatyana V, Mokrushina Yuliana A, Ponomarenko Natalia A, Belogurov Alexey A, Rubtsova Maria P, Kartseva Olga V, Gomzikova Marina O, Moskovtsev Alexey A, Bukatin Anton S, Dubina Michael V, Kostryukova Elena S, Babenko Vladislav V, Vakhitova Maria T, Manolov Alexander I, Malakhova Maja V, Kornienko Maria A, Tyakht Alexander V, Vanyushkina Anna A, Ilina Elena N, Masson Patrick, Gabibov Alexander G, Altman Sidney

Abstract excerpt

Ultrahigh-throughput screening (uHTS) techniques can identify unique functionality from millions of variants. To mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-oil-in-water emulsion (MDE). Biocompatible MDE enables in-droplet cultivation of different living...

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Identifiers

PMID: 28202731
DOI: 10.1073/pnas.1621226114
PMCID: PMC5347554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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