Article
A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe.
Nucleic acids research - 1 Mar 2011
Li Jun, Zhang Jia-Min, Li Xin, Suo Fang, Zhang Mei-Jun, Hou Wenru, Han Jinghua, Du Li-Lin
Abstract excerpt
The TTAA-specific transposon piggyBac (PB), originally isolated from the cabbage looper moth, Trichoplusia ni, has been utilized as an insertional mutagenesis tool in various eukaryotic organisms. Here, we show that PB transposes in the fission yeast Schizosaccharomyces pombe and leaves almost no footprints. We developed a PB-based mutagenesis system for S. pombe by constructing a strain with a selectable...
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