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Article

piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics.

Genetics - 1 Feb 2003

Horn Carsten, Offen Nils, Nystedt Sverker, Häcker Udo, Wimmer Ernst A

Abstract excerpt

Transposon mutagenesis provides a fundamental tool for functional genomics. Here we present a non-species-specific, combined enhancer detection and binary expression system based on the transposable element piggyBac: For the different components of this insertional mutagenesis system, we used widely applicable transposons and distinguishable broad-range transformation markers, which should enable this system to...

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Identifiers

PMID: 12618403
DOI: 10.1093/genetics/163.2.647
PMCID: PMC1462455

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