Article
A 'marker switch' approach for targeted mutagenesis of genes in Schizosaccharomyces pombe.
Yeast (Chichester, England) - 1 May 2003
MacIver Fiona H, Glover David M, Hagan Iain M
Abstract excerpt
The completion of the Schizosaccharomyces pombe genome sequencing project has led to a dramatic acceleration of gene characterization in this system. Once a gene has been identified, the challenge then comes in using reverse genetics to generate a range of mutants in this gene of interest so that the powerful genetics and wealth of genetic backgrounds available in Sz. pombe can be exploited to study the function...
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