Article
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex.
BMC biochemistry - 31 Dec 2010
Kim Ji Hun, Chang Tsz M, Graham Alison N, Choo K H A, Kalitsis Paul, Hudson Damien F
Abstract excerpt
BACKGROUND: Cell biologists face the need to rapidly analyse their proteins of interest in order to gain insight into their function. Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective antibodies have not yet been generated. AIM: To develop a method that simplifies the critical protein analytical steps...
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