Article
Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity.
PLoS genetics - 1 Jun 2011
Attaiech Laetitia, Olivier Audrey, Mortier-Barrière Isabelle, Soulet Anne-Lise, Granadel Chantal, Martin Bernard, Polard Patrice, Claverys Jean-Pierre
Abstract excerpt
Bacteria encode a single-stranded DNA (ssDNA) binding protein (SSB) crucial for genome maintenance. In Bacillus subtilis and Streptococcus pneumoniae, an alternative SSB, SsbB, is expressed uniquely during competence for genetic transformation, but its precise role has been disappointingly obscure. Here, we report our investigations involving comparison of a null mutant (ssbB(-)) and a C-ter truncation (ssbBΔ7)...
Topics
- Bacterial Proteins
- Chromosomes, Bacterial
- DNA, Bacterial
- DNA, Single-Stranded
- DNA-Binding Proteins
- Models, Biological
- Mutation
- Plasmids
- Streptococcus pneumoniae
- Transformation, Bacterial