Article
CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Schiml Simon, Fauser Friedrich, Puchta Holger
Abstract excerpt
The CRISPR/Cas system has recently become the most important tool for genome engineering due to its simple architecture that allows for rapidly changing the target sequence and its applicability to organisms throughout all kingdoms of life. The need for an easy-to-use and reliable nuclease is especially high in plant research, as precise genome modifications are almost impossible to achieve by...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.