Article
Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Lee Ze Hong, Yamaguchi Nobutoshi, Ito Toshiro
Abstract excerpt
Gene editing enables the modification of genetic information in a targeted manner. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a gene editing approach that has been widely used in both the animal and plant studies. Plant transcription factors play critical roles in plant development. Recent studies have used the CRISPER/Cas9 system to disrupt...
Topics
- Agrobacterium
- Arabidopsis
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- DNA Primers
- Genome, Plant
- Molecular Biology
- Mutagenesis
- Mutation
- Physical Chromosome Mapping
- RNA, Guide, CRISPR-Cas Systems