Article
High-throughput TILLING for Arabidopsis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Till Bradley J, Colbert Trenton, Codomo Christine, Enns Linda, Johnson Jessica, Reynolds Steven H, Henikoff Jorja G, Greene Elizabeth A, Steine Michael N, Comai Luca, Henikoff Steven
Abstract excerpt
Targeting induced local lesions in genomes (TILLING) is a general strategy for identifying induced point mutations that can be applied to almost any organism. In this chapter, we describe the basic methodology for high-throughput TILLING. Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence and its wild-type...
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