Article
Mutagenesis in Arabidopsis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Maple Jodi, Møller Simon G
Abstract excerpt
Ethyl methane sulfonate (EMS) mutagenesis in Arabidopsis is the most widely used mutagenesis technique. EMS has high mutagenicity and low mortality and can be used in any laboratory with a fume hood. The chemical principle of EMS mutagenesis is simple; it is based on the ability of EMS to alkylate guanine bases, which results in base mispairing. An alkylated guanine will pair with a thymine base, resulting...
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