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Article

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles.

Planta - 1 Jan 2015

Hyun Youbong, Kim Jungeun, Cho Seung Woo, Choi Yeonhee, Kim Jin-Soo, Coupland George

Abstract excerpt

MAIN CONCLUSION: Dividing tissue-targeted site-directed mutagenesis using RGEN of CRISPR/Cas system produces heritable mutations in Arabidopsis thaliana. Site-directed genome engineering in higher plants has great potential for basic research and molecular breeding. Here, we describe a method for site-directed mutagenesis of the Arabidopsis nuclear genome that efficiently generates heritable mutations using the...

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Identifiers

PMID: 25269397
DOI: 10.1007/s00425-014-2180-5
PMCID: PMC4282705

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