Article
CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1.
Genes & development - 15 Oct 2010
Bartkowiak Bartlomiej, Liu Pengda, Phatnani Hemali P, Fuda Nicholas J, Cooper Jeffrey J, Price David H, Adelman Karen, Lis John T, Greenleaf Arno L
Abstract excerpt
Drosophila contains one (dCDK12) and humans contain two (hCDK12 and hCDK13) proteins that are the closest evolutionary relatives of yeast Ctk1, the catalytic subunit of the major elongation-phase C-terminal repeat domain (CTD) kinase in Saccharomyces cerevisiae, CTDK-I. However, until now, neither CDK12 nor CDK13 has been demonstrated to be a bona fide CTD kinase. Using Drosophila, we demonstrate that dCDK12...
Topics
- Animals
- Blotting, Western
- CDC2 Protein Kinase
- Cell Line
- Chromosome Mapping
- Cyclin T
- Cyclin-Dependent Kinase 9
- Cyclin-Dependent Kinases
- Drosophila Proteins
- Drosophila melanogaster
- Genetic Complementation Test
- HeLa Cells
- Humans