Article
Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function.
Genetics - 1 Oct 1997
Kopski K M, Huffaker T C
Abstract excerpt
We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene that encodes the 110-kD subunit of the Saccharomyces cerevisiae CBF3 kinetochore complex. At the restrictive temperature of 37 degrees, ndc10-2 cells are able to assemble anaphase spindles, but fail to segre...
Topics
- Anaphase-Promoting Complex-Cyclosome
- Cloning, Molecular
- DNA-Binding Proteins
- Fungal Proteins
- Gene Dosage
- Genes, Lethal
- Genes, Suppressor
- Hot Temperature
- Kinetochores
- Ligases
- Phenotype
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Ubiquitin-Protein Ligase Complexes
- Ubiquitin-Protein Ligases
- Ubiquitins