Article
Nuclear retention of unspliced pre-mRNAs by mutant DHX16/hPRP2, a spliceosomal DEAH-box protein.
The Journal of biological chemistry - 12 Nov 2010
Gencheva Marieta, Lin Ting-Yu, Wu Xiwei, Yang Lixin, Richard Caroline, Jones Matthew, Lin Shwu-Bin, Lin Ren-Jang
Abstract excerpt
Defective or imbalanced expression of spliceosomal factors has been linked to human disease; however, how a defective spliceosome affects intron-containing gene transcripts in human cells is largely unknown. DEAH-box protein DHX16 is a human orthologue of Saccharomyces cerevisiae spliceosomal protein Prp2, an RNA-dependent ATPase that activates the spliceosome before the first catalytic step of splicing. Yeast...
Topics
- Amino Acid Substitution
- Blotting, Northern
- Blotting, Western
- Cell Nucleus
- Gene Expression Profiling
- HEK293 Cells
- Humans
- Introns
- Mutation
- Oligonucleotide Array Sequence Analysis
- RNA
- RNA Helicases
- RNA Precursors