The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.

doi:10.1016/s0960-9822(98)70178-2

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Article

The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.

Current biology : CB - 9 Apr 1998

Wang Y, Wagner J D, Guthrie C

Abstract excerpt

BACKGROUND: During pre-mRNA splicing, dynamic rearrangement of RNA secondary structure within the spliceosome is crucial for intron recognition and formation of the catalytic core. Splicing factors belonging to the DExD/DExH-box family of RNA-dependent ATPases are thought to have a central role i...

Topics

Adenosine Triphosphatases
Adenosine Triphosphate
Base Sequence
Fungal Proteins
Kinetics
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA
RNA Helicases
RNA Splicing
RNA Splicing Factors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Double-Stranded

RNA, Small Nuclear

Recombinant Fusion Proteins

Ribonucleotides

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Source

Open in PubMed

Identifiers

PMID: 9550699
DOI: 10.1016/s0960-9822(98)70178-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9550699-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.PMID 9550699Read ↗

Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase.Semantic proximity Read ↗

Plasmodium falciparum Prp16 homologue and its role in splicing.Semantic proximity Read ↗

The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes.Semantic proximity Read ↗

A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.Semantic proximity Read ↗

A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.Semantic proximity Read ↗

A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing.Semantic proximity Read ↗

Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing.Semantic proximity Read ↗

Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.Semantic proximity Read ↗

Brr2p carboxy-terminal Sec63 domain modulates Prp16 splicing RNA helicase.Semantic proximity Read ↗

Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing.Semantic proximity Read ↗

Nuclear retention of unspliced pre-mRNAs by mutant DHX16/hPRP2, a spliceosomal DEAH-box protein.Semantic proximity Read ↗

Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2.Semantic proximity Read ↗

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