Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase.

doi:10.1093/genetics/137.3.677

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Article

Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase.

Genetics - 1 Jul 1994

Madhani H D, Guthrie C

Abstract excerpt

Pre-mRNA splicing occurs in a large and dynamic ribonucleoprotein complex, the spliceosome. Several protein factors involved in splicing are homologous to a family of RNA-dependent ATPases, the so-called DEAD/DEAH proteins. A subset of these factors exhibit RNA helicase activity in vitro. The DEAD/DEAH proteins involved in splicing are thought to mediate RNA conformational rearrangements during spliceosome...

Topics

Adenosine Triphosphatases
Alleles
Base Sequence
Fungal Proteins
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Suppressor
Ligands
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleic Acid Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA Helicases

RNA Nucleotidyltransferases

RNA Precursors

RNA Splicing

RNA Splicing Factors

RNA, Small Nuclear

RNA-Binding Proteins

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Identifiers

PMID: 8088513
DOI: 10.1093/genetics/137.3.677
PMCID: PMC1206027

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8088513-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase.PMID 8088513Read ↗

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.Semantic proximity Read ↗

Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation.Semantic proximity Read ↗

Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing.Semantic proximity Read ↗

The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.Semantic proximity Read ↗

A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.Semantic proximity Read ↗

Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.Semantic proximity Read ↗

The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.Semantic proximity Read ↗

Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2.Semantic proximity Read ↗

Interactions of the yeast U6 RNA with the pre-mRNA branch site.Semantic proximity Read ↗

Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.Semantic proximity Read ↗

Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.Semantic proximity Read ↗

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.Semantic proximity Read ↗

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