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Article

DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast.

Nucleic acids research - 11 Jun 1991

Mellor J, Rathjen J, Jiang W, Barnes C A, Dowell S J

Abstract excerpt

The centromere and promoter factor 1 (CPF1) binds specifically in vitro and in vivo to an octanucleotide (RTCACRTG). This sequence is found in the centromere DNA element I (CDEI) of yeast centromeres and upstream from a number of transcription units including MET25, GAL2 and TRP1. Inactivation of the CPF1 gene results in three phenotypes; slow growth, a partial loss of centromere function and methionine...

Topics

Amino Acid Sequence
Autoradiography
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Blotting, Northern
Chromosomes, Fungal
DNA, Fungal
DNA-Binding Proteins
Fungal Proteins
Genes, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Methionine

Molecular Sequence Data

Mutation

Plasmids

Protein Biosynthesis

RNA, Fungal

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 2057354
DOI: 10.1093/nar/19.11.2961
PMCID: PMC328258

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2057354-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast.PMID 2057354Read ↗

Mutational analysis of the Saccharomyces cerevisiae general regulatory factor CP1.Semantic proximity Read ↗

Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophy.Semantic proximity Read ↗

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Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription.Semantic proximity Read ↗

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