Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1.

doi:10.1128/mcb.10.6.2458-2467.1990

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Article

Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1.

Molecular and cellular biology - 1 Jun 1990

Baker R E, Masison D C

Abstract excerpt

CP1 is a sequence-specific DNA-binding protein of the yeast Saccharomyces cerevisiae which recognizes the highly conserved DNA element I (CDEI) of yeast centromeres. We cloned and sequenced the gene encoding CP1. The gene codes for a protein of molecular weight 39,400. When expressed in Escherichia coli, the CP1 gene directed the synthesis of a CDEI-binding protein having the same gel mobility as purified yeast...

Topics

Amino Acid Sequence
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Centromere
Chromosomes, Fungal
DNA, Fungal
DNA-Binding Proteins
Escherichia coli
Fungal Proteins
Genes
Genes, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genotype

Immunoblotting

Molecular Sequence Data

Phenotype

Plasmids

Restriction Mapping

Saccharomyces cerevisiae

Source

Open in PubMed Open full text

Identifiers

PMID: 2188087
DOI: 10.1128/mcb.10.6.2458-2467.1990
PMCID: PMC360602

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2188087-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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