Article
Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-strand conformation polymorphism analysis.
Cancer research - 1 Jul 1991
Murakami Y, Hayashi K, Sekiya T
Abstract excerpt
Aberrations of the p53 gene and its transcript in ten human tumor-derived cell lines were investigated by single-strand conformation polymorphism analysis of polymerase chain reaction products. This method can detect loss of one of the two alleles and a point mutation in the remaining allele simultaneously. Aberrations were newly found in four tumor cell lines. Three cell lines had lost one of the alleles and had...
Topics
- Alleles
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Humans
- Molecular Sequence Data
- Nucleic Acid Conformation
- Oligonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic
- RNA, Messenger