Article
Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s).
Nucleic acids research - 11 Feb 1992
Danenberg P V, Horikoshi T, Volkenandt M, Danenberg K, Lenz H J, Shea L C, Dicker A P, Simoneau A, Jones P A, Bertino J R
Abstract excerpt
RNA molecules were found to separate into numerous metastable conformational forms upon non-denaturing gel electrophoresis. The equilibration of the conformations was accelerated by heating or mild denaturing conditions. Single-base substitutions in the sequence of the RNAs caused changes in the conformational patterns, including mobility shifts of major and minor conformations, appearance of new conformations...
Topics
- Base Sequence
- Cell Line
- DNA
- Electrophoresis
- Genes, p53
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic