Article
Analysis of p53 mutations in single cells obtained from histological tissue sections.
Analytical biochemistry - 1 Dec 2000
Persson A E, Ling G, Williams C, Bäckvall H, Pontén J, Pontén F, Lundeberg J
Abstract excerpt
We have previously reported on direct sequence analysis of the p53 gene in laser-dissected single cells from tissue sections, where each allele of two fragments (exons 7 and 8) could be accurately analyzed in only 14% of the cells due to the high frequency of exon and allele dropout. Here in an effort to minimize this problem, we have investigated various approaches for sample preparation and gene amplification....
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